A potent, selective inhibitor of UCH-L3
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TCID

Item No. 16353

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
4,5,6,7-tetrachloro-1H-indene-1,3(2H)-dione
CAS Number
30675-13-9
Synonyms
  • UCH-L3 Inhibitor
Molecular Formula
C9H2Cl4O2
Formula Weight
283.9
Purity
≥95%
Formulation
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 15 mg/mlDMSO: 10 mg/ml
λmax
244 nm
SMILES
O=C(CC1=O)C2=C1C(Cl)=C(Cl)C(Cl)=C2Cl
InChi Code
InChI=1S/C9H2Cl4O2/c10-6-4-2(14)1-3(15)5(4)7(11)9(13)8(6)12/h1H2
InChi Key
IDLAOWFFKWRNHB-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    5 mg

    $81

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    10 mg

    $128

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    25 mg

    $290

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    Product Description

    Ubiquitin carboxyl-terminal hydrolase isozyme L3 (UCH-L3) is a deubiquitinating enzyme and thiol-dependent hydrolase.1,2,3,4 TCID is a tetrachloro indandione that acts as a potent inhibitor of UCH-L3 and demonstrates 125-fold selectivity over UCH-L1 (IC50s = 0.6 and 75 µM, respectively).5

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Mason, D.E., Ek, J., Peters, E.C., et al. Substrate profiling of deubiquitin hydrolases with a positional scanning library and mass spectrometry. Biochemistry 43(21), 6535-6544 (2004).

    2. Setsuie, R., Sakurai, M., Sakaguchi, Y., et al. Ubiquitin dimers control the hydrolase activity of UCH-L3. Neurochem. Int. 54(5-6), 314-321 (2009).

    3. Wada, H., Kito, K., Caskey, L.S., et al. Cleavage of the C-terminus of NEDD8 by UCH-L3. Biochem. Biophys. Res. Commun. 251(3), 668-692 (1998).

    4. Iphöfer, A., Kummer, A., Nimtz, M., et al. Profiling ubiquitin linkage specificities of deubiquitinating enzymes with branched ubiquitin isopeptide probes. Chembiochem 13(10), 1416-1420 (2012).

    5. Liu, Y., Lashuel, H.A., Choi, S., et al. Discovery of inhibitors that elucidate the role of UCH-L1 activity in the H1299 lung cancer cell line. Chem. Biol. 10(9), 837-846 (2003).